Recombinant protein protocols : detection and isolation /
This handy bench-top manual provides detailed, step-by-step protocols for the detection and isolation of expressed proteins, in situ detection techniques for recombinant genes, and a unique section on the applications of recombinant gene expression covering such areas as the use of transgenic animal...
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| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
c c1997
Totowa, N.J. : c1997 Totowa, NJ : 1997 Totowa, NJ : c1997 Totowa, N.J. : [1997] |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
63 Methods in molecular biology (Clifton, N.J.) ; v. 63 Methods in molecular biology (Clifton, N.J.) 63 Methods in molecular biology (Clifton, N.J.) |
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Table of Contents:
- Detection and selection of cultured cells secreting recombinant product by soft agar cloning and antibody overlay / Marylou G. Gibson, Karmen Hodges, Lauretta Lowther
- Detection and isolation of recombinant protein based on binding affinity reporter. Maltose binding protein / Paul Riggs
- Detection and isolation of recombinant proteins based on binding affinity of reporter. Protein A / Stefan Ståhl, Per-Åke Nygren, Mathias Uhlén
- Expression and purification of recombinant streptavidin-containing chimeric proteins / Takeshi Sano, Cassandra L. Smith, Charles R. Cantor
- Bacterial expression, purification, and potential use of His-tagged GAL4 fusion proteins / M. Lienhard Schmitz, Patrick A. Baeuerle
- Detection of expressed recombinant protein based on multidrug resistance : P-glycoprotein / Ursula A. Germann
- Detection and isolation of recombinant proteins from mammalian cells by immunoaffinity chromatography : p53 / Jamil Momand, Bahman Sepehrnia
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- Methods for double detection of gene expression : combined in situ hybridization and immunocytochemistry or histochemistry / Ronald A. Conlon
- In situ PCR : current protocol / Omar Bagasra, Muhammad Mukhtar, Farida Shaheen, Roger J. Pomerantz
- Expression and detection of green fluorescent protein (GFP) / Steven R. Kain, Paul Kitts
- Transgenic animals as bioreactors for expression of recombinant proteins / Jaspal S. Khillan
- Artificial cells for bioencapsulation of cells and genetically engineered E. coli : for cell therapy, gene therapy, and removal of urea and ammonia / Thomas M. Chang, Satya Prakash
- Hyperexpression of a synthetic protein-based polymer gene / Henry Daniell, Chittibabu Guda, David T. McPherson, Xiaorong Zhang, Jie Xu, Dan W. Urry
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- Overview of experimental strategies on the detection and isolation of recombinant proteins and their applications / Rocky S. Tuan
- Reporter systems / Debyra Groskreutz, Elaine T. Schenborn
- Detection of recombinant protein based on reporter enzyme activity : chloramphenicol acetyltransferase / Peiyu Lee, Dennis E. Hruby
- Human placental alkaline phosphatase as a marker for gene expression / Paul Bates, Michael H. Malim
- Use of secreted alkaline phosphatase as a reporter of gene expression in mammalian cells / Steven R. Kain
- Detection of [beta]-galactosidase and [beta]-glucuronidase using chemiluminescent reporter gene assays / Corinne E. Olesen, John J. Fortin, John C. Voyta, Irena Bronstein
- Chemiluminescent immunoassay for the detection of chloramphenicol acetyltransferase and human growth hormone reporter proteins / Corinne E. Olesen, John C. Voyta, Irena Bronstein
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- Transplantation of cells in an immunoisolation device for gene therapy / Victoria E. Carr-Brendel, Robin L. Geller, Tracy J. Thomas, Daniel R. Boggs, Susan K. Young, JoAnne Crudele, Laura A. Martinson, David A. Maryanov, Robert C. Johnson, James H. Brauker
- Delivery of recombinant HIV-1-directed antisense and ribozyme genes / Georg Sczakiel, Giorgio Palú, William James
- Inducible system designed for future gene therapy / Yaolin Wang, Bert W. O'Malley, Sophia Y. Tsai
- Human gene therapy : dreams to realization / Muhammad Mukhtar, Zahida Parveen, Omar Bagasra
- Yeast GAL4 two-hybrid system : a genetic system to identify proteins that interact with a target protein / Li Zhu
- Alternative yeast two-hybrid systems : the interaction trap and interaction mating / Erica A. Golemis, Vladimir Khazak
- Detection of heterologous Gs-coupled receptor activity in LLC-PK1 cells based on expression of urokinase-type plasminogen activator / Luigi Catanzariti, Brian A. Hemmings
- Histochemical and fluorochrome-based detection of [beta]-galactosidase / Ruth Sullivan, Cecilia W. Lo
- Combined, sequential in situ hybridization and immunohistochemistry on the same tissue section / Kenneth J. Shepley, Rocky S. Tuan
- Whole mount in situ hybridization to embryos and embryonic tissues / Ronald A. Conlon
- Whole-mount in situ hybridization for developing chick embryos using digoxygenin-labeled RNA probes / Christopher W. Hsu, Rocky S. Tuan
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