Handbook of ELISPOT : methods and protocols /
Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccin...
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| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
©2012
New York : Humana Press : Imprint: Humana Press, c2012 Totowa, NJ : 2012 |
| Edition: | 2 |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 792 Methods in molecular biology (Clifton, N.J.) ; volume 792 Methods in molecular biology 792 Springer Protocols (Springer-12345) Springer protocols (Series) Springer protocols |
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Table of Contents:
- Unique Strengths of ELISPOT for T Cell Diagnostics
- The Impact of Harmonization on ELISPOT Assay Performance
- Equine ELISPOT Assay to Study Secretion of IFNγ and IL-4 from Peripheral Blood Mononuclear Cells
- Utilization of Feline ELISPOT for Mapping Vaccine Epitopes
- Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay
- Dual and Triple Color Fluorospot
- ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes
- ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells
- ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion
- Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell
- How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells' Secretory Activity
- Mathematical Algorithms for Automatic Search, Recognition, and Detection of Spots in ELISPOT Assay
- Objective, User-Independent ELISPOT Data Analysis Based on Scientifically Validated Principles
- Statistical Analysis of ELISPOT Assays
- Response Determination Criteria for ELISPOT: Toward a Standard that Can Be Applied Across Laboratories
- Detection of Vaccinia Virus-Specific IFNγ and IL-10 Secretion from Human PBMCs and CD8+ T Cells by ELISPOT
- ELISPOT Assays to Enumerate Bovine IFN-γ Secreting Cells for the Development of Novel Vaccines Against Bovine Tuberculosis
- IL-7 Addition Increases Spot Size and Number as Measured by T-SPOT.TB®
- Overview of Membranes and Membrane Plates Used in Research and Diagnostic ELISPOT Assays
- Unique strengths of ELISPOT for T cell diagnostics
- The impact of harmonization on ELISPOT assay performance
- Equine ELISPOT assay to study secretion of IFN[gamma] and IL-4 from peripheral blood mononuclear cells
- Utilization of feline ELISPOT for mapping vaccine epitopes
- Analyzing cellular immunity to AAV in a canine model using ELISPOT assay
- Dual- and triple-color fluorospot
- ELISPOT assay as a tool to study oxidative stress in lymphocytes
- ELISPOT assay for neuroscience research: Studying TNT[alpha] secretion from microglial cells
- ELISPOT assay as a tool to study the effects of stem cells on cytokine secretion
- Combining ELISPOT and ELISA to measure amounts of cytokines secreted by a single cell
- How ELISPOT morphology reflects on the productivity and kinetics of cells' secretory activity
- Mathematical algorithms for automatic search, recognition, and detection of spots in ELISPOT assay
- Objective, user-independent ELISPOT data analysis based on scientifically validated principles
- Statistical analysis of ELISPOT assays
- Response determination criteria for ELISPOT: Toward a standard that can be applied across laboratories
- Detection of vaccinia virus-specific IFN[gamma] and IL-10 secretion from human pbmcs and CD8(+) T cells by ELISPOT
- ELISPOT assays to enumerate bovine IFN-[gamma]-secreting cells for the development of novel vaccines against bovine tuberculosis
- Il-7 addition increases spot size and number as measured by T-SPOT. TB
- Overview of membranes and membrane plates used in research and diagnostic ELISPOT assays
- ^^ Detection of Vaccinia virus-specific IFN[gamma] and IL-10 secretion from human PBMCs and CD8⁺ T cells by ELISPOT / Benjamin J. Umlauf ... [et al.]
- ELISPOT assays to enumerate bovine IFN-[gamma]-secreting cells for the development of novel vaccines against bovine tuberculosis / Martin Vordermeier and Adam O. Whelan
- IL-7 addition increases spot size and number as measured by T-SPOT.TB® / Marsha L. Feske ... [et al.]
- Overview of membranes and membrane plates used in research and diagnostic ELISPOT assays / Alan J. Weiss
- ^^ ELISPOT assay as a tool to study the effects of stem cells on cytokine secretion / Jun-Seop Shin and Chung-Gyu Park
- Combining ELISPOT and ELISA to measure amounts of cytokines secreted by a single cell / Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
- How ELISPOT morphology reflects on the productivity and kinetics of cells' secretory activity / Alexey Y. Karulin and Paul V. Lehmann
- Mathematical algorithms for automatic search, recognition, and detection of spots in ELISPOT assay / Sergey S. Zadorozhny and Nikolai N. Martynov
- Objective, user-independent ELISPOT data analysis based on scientifically validated principles / Wenji Zhang and Paul V. Lehmann
- Statistical analysis of ELISPOT assays / Marcus Dittrich and Paul V. Lehmann
- Response determination criteria for ELISPOT : toward a standard that can be applied across laboratories / Zoe Moodie ... [et al.]
- ^
- Unique strengths of ELISPOT for T cell diagnostics / Paul V. Lehmann and Wenji Zhang
- The impact of harmonization on ELISPOT assay performance / Sylvia Janetzki and Cedrik M. Britten
- Equine ELISPOT assay to study secretion of IFN[gamma] and IL-4 from peripheral blood mononuclear cells / Jodi Hagen ... [et al.]
- Utilization of feline ELISPOT for mapping vaccine epitopes / Jeffrey R. Abbott ... [et al.]
- Analyzing cellular immunity to AAV in a canine model using ELISPOT assay / Zejing Wang ... [et al.]
- Dual- and triple-color fluorospot / Niklas Ahlborg and Bernt Axelsson
- ELISPOT assay as a tool to study oxidative stress in lymphocytes / Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
- ELISPOT assay for neuroscience research : studying TNF[alpha] secretion from microglial cells / Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
- ^