Handbook of Human Oocyte Cryopreservation

An indispensable practical tool for embryologists and reproductive medicine specialists seeking to offer their patients this revolutionary treatment option

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Bibliographic Details
Main Author: Porcu, Eleonora
Other Authors: Ciotti, Patrizia, Venturoli, Stefano
Format: Book
Language:English
Published: Cambridge : Cambridge University Press, 2012
Subjects:
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100 1 |a Porcu, Eleonora 
245 1 0 |a Handbook of Human Oocyte Cryopreservation 
260 |a Cambridge :  |b Cambridge University Press,  |c 2012 
300 |a 1 online resource (160 pages) 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
500 |a Volume, temperature and time of exposure during rapid thawing 
504 |a Includes bibliographical references and index 
505 0 |a Chapter 1 Why freeze human eggs? Clinical and biological indications for human oocyte cryopreservation; Ethical and legal indications; Fertility-saving indications; IVF laboratory flexibility indication; The controversial family planning indication; Chapter 2 The evolution of human oocyte cryopreservation; Preliminary research; Early animal studies; Early human studies; Subsequent research; Recent clinical application; Safety of oocyte cryopreservation; Chapter 3 Clinical management of oocyte cryopreservation; Ovarian stimulation and oocyte storage 
505 8 |a Evolution of the slow freezing methodologySlow freezing/rapid thawing; Materials; Solutions for slow freezing; Thawing solutions for human oocytes; Setting up for a freezing cycle; Setting up for a thawing cycle; Freezing solutions at different sucrose concentrations; Thawing solutions with differentiated sucrose concentrations; Setting up for a freezing cycle with differential sucrose concentrations; Setting up for a thawing cycle with differential sucrose concentrations; Recipe formula for Na depleted PBS 
505 8 |a Storage of surplus eggs in a standard cycleEgg storage to avoid ovarian hyperstimulation syndrome; Egg storage in polycystic ovary syndrome; Egg storage in cancer patients; Chapter 4 Laboratory management of oocyte cryopreservation; The egg freezing laboratory: location, equipment, instruments, materials; Selecting freezable eggs: the freezable egg score; Evaluation of oocyte maturity and quality; Evaluation of cumulus-corona-oocyte complex maturity; Methodology; Evaluation of the nucleus; Evaluation of the cytoplasm; Cytoplasmic abnormalities; Extracytoplasmic abnormalities 
505 8 |a Volume, temperature and time of exposure during slow freezing utilizing solutions with 0.1 or 0.2 or 0.3M sucrose at freezing and thawing (freezing 0.1M/thawing 0.1M-freezing 0.2M/thawing 0.2M-freezing 0.3M/thawing 0.3M)Volume, temperature and time of exposure during slow freezing utilizing solutions with differentiated sucrose concentrations (freezing 0.2M sucrose/thawing 0.3M); Starting the freezing cycle; Loading the straw into the programmable freezer; Beginning of the freezing program; Seeding; Storage of the samples; Automated computerized freezing machine; Rapid thawing; Methodology 
505 8 |a Zona pellucida (ZP)Perivitelline space (PVS); Perivitelline space granules; First polar body fragmentation; Evaluation of the meiotic spindle; Grading of the oocyte; The freezable egg score; Survived oocyte; Degenerated first polar body; Degenerated oocyte; Oocyte with some sign of degeneration; Steps and timing of the entire cryopreservation procedure; Timing of the events that occur after HCG administration until embryo transfer; Timing of semen sample production; Organizing criteria for storage in liquid nitrogen tanks; Cryobiology; Chapter 5 Slow freezing; Principles 
520 |a An indispensable practical tool for embryologists and reproductive medicine specialists seeking to offer their patients this revolutionary treatment option 
588 0 |a Print version record 
650 0 |a Cryopreservation of organs, tissues, etc 
650 0 |a Oocytin  |x Cytology 
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650 7 |a SCIENCE  |x Life Sciences  |x General  |2 bisacsh 
650 1 2 |a Cryopreservation  |x methods 
650 1 2 |a Oocytes  |x cytology 
700 1 |a Ciotti, Patrizia 
700 1 |a Venturoli, Stefano 
776 0 8 |i Print version:  |a Porcu, Eleonora  |t Handbook of Human Oocyte Cryopreservation.  |d Cambridge : Cambridge University Press, ©2012   |z 9780521192392 
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