Handbook of Human Oocyte Cryopreservation
An indispensable practical tool for embryologists and reproductive medicine specialists seeking to offer their patients this revolutionary treatment option
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Format: | Book |
Language: | English |
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Cambridge :
Cambridge University Press,
2012
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072 | 7 | |a SCI |x 008000 |2 bisacsh | |
072 | 7 | |a SCI |x 086000 |2 bisacsh | |
082 | 0 | 4 | |a 570.752 |
100 | 1 | |a Porcu, Eleonora | |
245 | 1 | 0 | |a Handbook of Human Oocyte Cryopreservation |
260 | |a Cambridge : |b Cambridge University Press, |c 2012 | ||
300 | |a 1 online resource (160 pages) | ||
336 | |a text |b txt |2 rdacontent | ||
337 | |a computer |b c |2 rdamedia | ||
338 | |a online resource |b cr |2 rdacarrier | ||
500 | |a Volume, temperature and time of exposure during rapid thawing | ||
504 | |a Includes bibliographical references and index | ||
505 | 0 | |a Chapter 1 Why freeze human eggs? Clinical and biological indications for human oocyte cryopreservation; Ethical and legal indications; Fertility-saving indications; IVF laboratory flexibility indication; The controversial family planning indication; Chapter 2 The evolution of human oocyte cryopreservation; Preliminary research; Early animal studies; Early human studies; Subsequent research; Recent clinical application; Safety of oocyte cryopreservation; Chapter 3 Clinical management of oocyte cryopreservation; Ovarian stimulation and oocyte storage | |
505 | 8 | |a Evolution of the slow freezing methodologySlow freezing/rapid thawing; Materials; Solutions for slow freezing; Thawing solutions for human oocytes; Setting up for a freezing cycle; Setting up for a thawing cycle; Freezing solutions at different sucrose concentrations; Thawing solutions with differentiated sucrose concentrations; Setting up for a freezing cycle with differential sucrose concentrations; Setting up for a thawing cycle with differential sucrose concentrations; Recipe formula for Na depleted PBS | |
505 | 8 | |a Storage of surplus eggs in a standard cycleEgg storage to avoid ovarian hyperstimulation syndrome; Egg storage in polycystic ovary syndrome; Egg storage in cancer patients; Chapter 4 Laboratory management of oocyte cryopreservation; The egg freezing laboratory: location, equipment, instruments, materials; Selecting freezable eggs: the freezable egg score; Evaluation of oocyte maturity and quality; Evaluation of cumulus-corona-oocyte complex maturity; Methodology; Evaluation of the nucleus; Evaluation of the cytoplasm; Cytoplasmic abnormalities; Extracytoplasmic abnormalities | |
505 | 8 | |a Volume, temperature and time of exposure during slow freezing utilizing solutions with 0.1 or 0.2 or 0.3M sucrose at freezing and thawing (freezing 0.1M/thawing 0.1M-freezing 0.2M/thawing 0.2M-freezing 0.3M/thawing 0.3M)Volume, temperature and time of exposure during slow freezing utilizing solutions with differentiated sucrose concentrations (freezing 0.2M sucrose/thawing 0.3M); Starting the freezing cycle; Loading the straw into the programmable freezer; Beginning of the freezing program; Seeding; Storage of the samples; Automated computerized freezing machine; Rapid thawing; Methodology | |
505 | 8 | |a Zona pellucida (ZP)Perivitelline space (PVS); Perivitelline space granules; First polar body fragmentation; Evaluation of the meiotic spindle; Grading of the oocyte; The freezable egg score; Survived oocyte; Degenerated first polar body; Degenerated oocyte; Oocyte with some sign of degeneration; Steps and timing of the entire cryopreservation procedure; Timing of the events that occur after HCG administration until embryo transfer; Timing of semen sample production; Organizing criteria for storage in liquid nitrogen tanks; Cryobiology; Chapter 5 Slow freezing; Principles | |
520 | |a An indispensable practical tool for embryologists and reproductive medicine specialists seeking to offer their patients this revolutionary treatment option | ||
588 | 0 | |a Print version record | |
650 | 0 | |a Cryopreservation of organs, tissues, etc | |
650 | 0 | |a Oocytin |x Cytology | |
650 | 7 | |a Cryopreservation of organs, tissues, etc |2 fast | |
650 | 7 | |a NATURE |x Reference |2 bisacsh | |
650 | 7 | |a SCIENCE |x Life Sciences |x Biology |2 bisacsh | |
650 | 7 | |a SCIENCE |x Life Sciences |x General |2 bisacsh | |
650 | 1 | 2 | |a Cryopreservation |x methods |
650 | 1 | 2 | |a Oocytes |x cytology |
700 | 1 | |a Ciotti, Patrizia | |
700 | 1 | |a Venturoli, Stefano | |
776 | 0 | 8 | |i Print version: |a Porcu, Eleonora |t Handbook of Human Oocyte Cryopreservation. |d Cambridge : Cambridge University Press, ©2012 |z 9780521192392 |
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