Experimental models of cardiovascular diseases : methods and protocols /

This book provides methodological information on establishing reliable and reproducible experimental models of cardiovascular diseases. Chapters detail practical protocols from expert laboratories focusing on cardiovascular research, leading to novel discoveries in cardiac biology, and the developme...

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Bibliographic Details
Other Authors: Ishikawa, Kiyotake (Editor)
Format: Book
Language:English
Published: New York : Humana Press : Springer, [2018]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1816
Methods in molecular biology (Clifton, N.J.) v. 1816
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Table of Contents:
  • Part I: Overview 1. Experimental Models of Cardiovascular Diseases
  • Overview
  • Jae Gyun Oh and Kiyotake Ishikawa Part II :In Silico Models 2. An Introduction to Computational Modeling of Cardiac Electrophysiology and Arrhythmogenicity Joshua Mayourian, Eric A. Sobie, and Kevin D. Costa Part III: In Vitro Models 3. Isolation of Atrial and Ventricular Cardiomyocytes for In Vitro Studies Jelena Plačkić and Jens Kockskämper 4. Cardiomyocyte Differentiation from mouse Embryonic Stem Cells Adam T Lynch, Silvia Mazzotta, and Stefan Hoppler 5. Cardiomyocyte Differentiation from Human Embryonic Stem Cells Silvia Mazzotta, Adam T Lynch, and Stefan Hoppler 6. Induction of Human Induced Pluripotent Stem Cells to Cardiomyocytes using Embryoid Bodies Takeshi Hatani1, Kenji Miki, and Yoshinori Yoshida 7. Measuring Cardiomyocyte Contractility and Calcium Handling In Vitro Przemek A. Gorski, Changwon Kho, and Jae Gyun Oh Part IV: Ex Vivo Models 8
  • Part I: Overview 1. Experimental Models of Cardiovascular Diseases
  • Overview
  • Jae Gyun Oh and Kiyotake Ishikawa Part II :In Silico Models 2. An Introduction to Computational Modeling of Cardiac Electrophysiology and Arrhythmogenicity Joshua Mayourian, Eric A. Sobie, and Kevin D. Costa Part III: In Vitro Models 3. Isolation of Atrial and Ventricular Cardiomyocytes for In Vitro Studies Jelena Plačkić and Jens Kockskämper 4. Cardiomyocyte Differentiation from mouse Embryonic Stem Cells Adam T Lynch, Silvia Mazzotta, and Stefan Hoppler 5. Cardiomyocyte Differentiation from Human Embryonic Stem Cells Silvia Mazzotta, Adam T Lynch, and Stefan Hoppler 6. Induction of Human Induced Pluripotent Stem Cells to Cardiomyocytes using Embryoid Bodies Takeshi Hatani1, Kenji Miki, and Yoshinori Yoshida 7. Measuring Cardiomyocyte Contractility and Calcium Handling In Vitro Przemek A. Gorski, Changwon Kho, and Jae Gyun Oh Part IV: Ex Vivo Models 8